In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide

In Vitro Comparative Skin Irritation Induced by Nano and Non-Nano Zinc Oxide

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This study was designed to determine whether nano-sized ZnO has the potential to cause acute cutaneous irritation using cultured HaCaT keratinocytes and a human skin equivalent as in vitro models, compared to non-nanomaterials.Commercial nano ZnO with different sizes (50 nm and 100 nm) was characterized by dynamic light scattering (DLS) Siemens IQ-500 MB557G5S0B Built In Double Oven - Stainless Steel and microscopy (SEM) in different media.Nano ZnO reduced the cell viability Bed Sheets of HaCaT in a dose-dependent and time-dependent manner, in a similar way to macro ZnO.However, the 3D-epidermis model revealed no irritation at 1 mg/mL after 24 h of exposure.In conclusion, nano-sized ZnO does not irritate skin, in a similar manner to non-nano ZnO.